The XDY-2LED inverted fluorescence microscope consists of a fluorescence excitation microscopy system and an inverted biological microscopy system. It adopts an excellent infinite optical system and is equipped with a long working distance flat field objective lens and a large field eyepiece. The compact and stable high rigidity body fully embodies the anti vibration requirements of microscopic operations. The rotating swing in swing out spotlight system can be used to observe uncontaminated cultured cells in high culture dishes or cylindrical flasks. The fluorescence system adopts LED fluorescence excitation module, and the light source and color filter group adopt a linked design, making the switching between different color filter groups faster, safer, and more effective. The product can be used for microscopic observation of cellular tissues and transparent liquid tissues, as well as for fluorescence microscopy observation in fields such as biopharmaceuticals, medical testing, and disease prevention.